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KMID : 0900919900140030199
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Korean journal of Animal Reproduction
1990 Volume.14 No. 3 p.199 ~ p.204
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Preservation of Extended Thoroughbred Semen at Low Temperature
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Koh Tae-Hyuk
Lee Sang-Ho
Song Hai-Bum
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Abstract
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Equine semen was analysed for its survival after storage under different conditions. Total 12 ejaculates from 2 Thoroughbred were analysed for general characterisitcs and preservation at low temperature. The sperm fraction, concentration, nd the rate of motile spermatozoa were 57.91ml per ejaculate, 2.18times108/ml and 74.1%, respectively. The survival rate of spermatozoa was highest when diluted semen with E-Z Mixin was stored at 7~8^{circ}C. The optimum survival rate(>54%) can be obtained upto 24h at 7~8^{circ}C. However only 10% spermatozoa survived after 5h storage at 7~8^{circ}C without use of E-Z Mixin. Other ranges of temperature(15^{circ}C and room temperature) gave less survival rates(<25%). These results indicate that the extender could be used as a basic solution for the preservation of equine spermatozoa at low temperature. It also provides a practical method for short-term storage of collected equine semen in a simple manner.
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